KMID : 0525720130180030184
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Journal of Chitin and Chitosan 2013 Volume.18 No. 3 p.184 ~ p.189
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Effect of Deep Sea Water Containing Chitosan Oligosaccharides on Glucose Uptake in 3T3-L1 Adipocytes
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Shin Eun-Ji
Park Jung-Eun Ha Byung-Geun So Myung-Suk Shon Yun-Hee
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Abstract
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To investigate the effect of deep sea water (DSW) containing chitosan oligosaccharides (COS) on glucose uptake, we performed glucose uptake assay, RT-PCR and western blot in mature 3T3-L1 adipocytes with the treatment of DSW or DSW containing COS. Both DSW and DSW containing COS treatment increased glucose uptake in mature 3T3-L1 adipocytes. RT-PCR results revealed that the expressions of genes related to glucose uptake such as GLUT 4 for glucose transporter, IRAP for an abundant cargo protein of GLUT 4 storage vesicles (GSVs), Syntaxin 6, and Syntaxin16 for vesicle trafficking were increased. Moreover, the phosphorylation of IRS 1, which is an important signal molecule for insulin-dependent glucose uptake, was increased by DSW and DSW containing COS treatment in mature 3T3-L1 adipocytes. In conclusion, these results suggest that DSW containing COS is more effective than DSW alone in stimulatory effect on glucose uptake.
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KEYWORD
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Deep sea water, Chitosan oligosaccharide, Glucose uptake, Glucose transporter 4
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